Objectives: The ethyl acetate fraction of bark and leaf part of Homalium zelanicum showed potential antioxidant and hepatoprotective activities. So, this study is aimed to evaluate antioxidant and hepatoprotective activities of hydroalcohol extract of Homalium zelanicum in carbon tetrachloride induced hepatotoxicity. Methods: To evaluate antioxidant potential, in vitro antioxidant assays e.g. DPPH, superoxide, hydroxyl and nitric oxide and ex vivo antioxidant assay e.g. cell based antioxidant protection in erythrocytes (CAP-e) were performed. Hepatoprotective activities were evaluated by estimating biochemical parameters such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total bilirubin (TB) and total protein (TP). Hepatic markers such as thiobarbituric acid reactive species (TBARS), superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) were evaluated. Results: Both bark and leaf of Homalium zeylanicum showed improvement in antioxidant protection of hepatocytes. As well as the extracts exhibited significant (P<0.001) hepatoprotection against CCl4-induced liver injury. Conclusion: Among all the extracts, hydroalcohol extract of bark of Homalium zeylanicum (HZB, 400 mg/kg) showed significant improvement in restoring serum and hepatic markers and the results were supported with histopathological observations.
Key words: Antioxidant, CCl4, Hepatic marker, CAP-e, Homalium zeylaicum.