Objective : To determine the amikacin sulfate by developing a new spectrophotometric method by derivatization using double beam spectrophotometer from pure and commercial brands. Method: A quantitative analytical method was developed by amikacin sulfate using spectrophotometer after derivatization with vanillin under optimized parameters like pH, heating time and temperature, volume of reagent, Impact of mixing order, Effect of addition of solvents and Effect of excipients. The method was successfully applied on bulk and different brands containing amikacin sulfate. Results: The bulk and pharmaceutical analysis was carried out of different brands with formation of slight yellow colored imine base. The product showed absorbance at 400 nm with molar absorptivity of 5.27×103 L/mole/cm. In a concentration of 10-50µg ml-1 a linear relationship was established with absorbance which follow the beer’s law with coefficient of determination r2 0.9991-0.9998. The procedure was valid because it did not show change in absorbance of the derivative up to 3 days. The sandells sensitivity was calculated as 0.004 at 0.45 µg mL-1 of amikacin sulfate with vanillin. The percentage of recovery was 103-106.4 % with RSD of 0.004-0.005.
Key words: Amikacin Sulfate, Vanillin, Spectrometric, Pakistan.
