More than 300 constituents have been identiÞ ed in different propolis samples. This study investigated the composition of ethanolic extracts of propolis samples collected from the Gujarat zone. A gas chromatographymass spectrometry was carried out on a Agilent GC-MS 5975 under electron impact ionization (70 eV). The chromatographic column for the analysis was done by the HP5MS capillary column (30 m x 0.25 mm internal diameter). The carrier gas used was helium at a ß ow rate of 1 ml/min. The oven temperature was 100°C to 280°C with a constant increase of 10°C. Propolis samples were analyzed with the column held initially at 60°C for 2 min and then increased to 230°C with a 2°C/ min heating ramp and then kept at 230°C for 3 min. Finally, the temperature was increased to 280°C with a 3°C/min heating ramp. The injection was performed in split mode at 220°C. Sample volumes of 1 μl were injected and analyzed by GC-MS. The following compounds were identiÞ ed for the Þ rst time in the propolis sample: p-coumeric acid, Benzyl cinnamate, 4-pentanoic acid, and Ferulic acid. The main type of the compound identiÞ ed was fatty acids derivatives. Similar results were found by Silici, et al.[5] for the turkey propolis.
Key words: GC-MS analysis, propolis, Honeycomb.
