Home Pharmacognosy In vivo Antidiabetic and Antioxidant Potential of Stephania hernandifolia in Streptozotocin-Induced-Diabetic Rats

In vivo Antidiabetic and Antioxidant Potential of Stephania hernandifolia in Streptozotocin-Induced-Diabetic Rats

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Published on: July 2010

Journal of Young Pharmacists, 2010; 2(3):255-260

Pharmacognosy | doi:10.4103/0975-1483.66803
Authors:

Sharma U, Sahu RK1, Roy A2, Golwala DK3

Department of Pharmacognosy, Institute of Pharmacy, Jalpaiguri, West Bengal,

1Oriental College of Pharmacy, Bhopal, Madhya Pradesh,

 2Department of Pharmacology, Columbia College of Pharmacy, Mandhar, Raipur, Chhattisgarh, India.

3C.U. Shah College of Pharmacy and Research, Surendranagar- Ahmedabad Highway, Wadhwan, Gujarat,

Abstract:

Stephania hernandifolia (Menispermaceae) is a medicinal plant, used by herbalists for treating various diseases, one of which is diabetes mellitus, in Darjeeling. However, its antidiabetic activity has not been scientifically investigated so far. The aim of this study, therefore, is to investigate the antidiabetic and antioxidant potential of the powdered corm of Stephania hernandifolia. This was tested in normal and Streptozotocin (STZ)-induced diabetic rats, using oral administration of ethanol and an aqueous extract (400 mg/kg body weight) of Stephania hernandifolia corm. After the oral administration of water and ethanol extracts at doses of 400 mg/kg body weight, blood glucose levels were monitored at specific intervals and it was found that they were significant lowered. Glibenclamide was used as a standard drug at a dose of 0.25 mg/kg. The experimental data revealed that both extracts has significant antihyperglycemic and antioxidant activity in Streptozotocin-induced rats compared to the standard drug. The antioxidant activity in vitro was measured by means of the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and Superoxide-free radical scavenging assay. Ascorbic acid, a natural antioxidant, was used as a control. The extracts of ethanol and aqueous were strongly scavenged DPPH radicals, with IC50 being 265.33 and 217.90 μg/ml, respectively. Although the extracts of ethanol and aqueous were moderately scavenged, the superoxide radical were with IC50 values of 526.87 and 440.89 μg/ml. The study revealed that the ethanolic extract exhibited more significant antidiabetic and antioxidant activity then the aqueous extract.

Key words: Antidiabetic, antioxidant, glibenclamide, lipid profile, Stephania hernandifolia, Streptozotocin.