Isolation, Characterization and Assessment for Muscle Relaxant Activity of Novel Phytomolecule from Galphimia glauca Cav. Stems

Baba Shankar Rao Garige*, Dugur Prasanna Laxmi, Donthuri Akhila Sravani, Jillella Rachana, Gaddam Shirisha, Ram Mohan Manda, Vasudha Bakshi

Department of Pharmacognosy and Phytochemistry, School of Pharmacy, Anurag University, Medchal District, 501 301, Telangana, INDIA.

Abstract:

Objectives: Galphimia glauca Cav (Gg) is an evergreen medicinal herb found growing in Indian subcontinent and more in the Deccan plateau regions of south India. The objective of the current work is to isolate, characterize and explore the muscle relaxant activity of the novel isolated phytomolecule (BS-4) from G. glauca stems using in vivo models. Methods: The obtained bio-active fraction (methanol fraction) of the G. glauca stem methanol extract (GgSME) was subjected to column chromatography and Preparative thin layer chromatography (TLC) to isolate the bioactive phytomolecule. The novel isolated bioactive phytomolecule was coded as “BS-4”, it is then characterized by R_f value, melting point, IR spectra, HPLC, Mass spectra, ¹H-NMR spectrum and ¹³C NMR spectrum and evaluated for muscle relaxant activity by administering BS-4 in Swiss albino mice in one day to animal models like Rota rod test and Grip strengthening test. Results: The LD₅₀ of BS-4 was > 2000 mg/kg. The mice were treated with BS-4 at 12.5, 25 and 50mg/kg doses. The BS-4 showed dose dependent significant (P ≤ 0.001) effects on muscle relaxant activity in Rota rod test and Grip strengthening test in mice (P ≤ 0.001). Conclusion: The current study results conclude that the isolated novel phytomolecule BS-4 has significant muscle relaxant effects.

Keywords: Galphimia glauca Clav, Column chromatography, Rota rod test, Grip strengthening test, Phytomolecule.