In vitro Immunomodulatory Effect from Edible Green Seaweed of Caulerpa lentillifera Extracts on Nitric Oxide Production and Phagocytosis Activity of RAW 264.7 Murine Macrophage Cells

    Published on:December 2020
    Journal of Young Pharmacists, 2020; 12(4):334-337
    Original Article | doi:10.5530/jyp.2020.12.87

    Sofa Fajriah1,*, Sri Handayani2, Ellya Sinurat3, Megawati Megawati1, Akhmad Darmawan1, Hariyanti Hariyanti4, Rizna Triana Dewi1, Abdi Wira Septama1

    1Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan PUSPIPTEK Serpong, Kota Tangerang Selatan, Banten, INDONESIA.

    2Research Unit for Natural Product Technology, Indonesian Institute of Sciences, Jl. Jogja-Wonosari, Gunung Kidul, Yogyakarta, INDONESIA.

    3Research and Development Center for Marine and Fisheries Product Processing and Biotechnology, Ministry of Marine Affairs and Fisheries RI, Jl KS Tubun Petamburan VI, Slipi, Central Jakarta, INDONESIA.

    4Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, Kebayoran Baru, Jakarta, INDONESIA.


    Background: Caulerpa lentillifera is edible macroalgae which has several biological properties. This marine plant also contains beneficial secondary metabolites that can be used for treatment and prevent several deficiencies. Objectives: This research was conducted to evaluate immunomodulatory effect on nitric oxide production and phagocytosis of RAW 264.7 murine macrophage cells from edible green seaweed of C. lentillifera extract. Methods: Extraction was applied to obtained ethanol extract, water extract and insoluble fraction. Alamar blue inclusion method was used to determine cytotoxicity and proliferation of RAW 264.7 after treated with sample. Griess assay using colourimetric commercial kits was selected to evaluate NO production and phagocytosis effect of samples was determined using phagocytic kit. Results: C. lentillifera extracts did not show any cytotoxic effect against RAW 264.7 macrophage. One hundred μg/mL of water extract increased the production of nitrite oxide. This extract revealed suspected-giant cells formation as a results of phagocytosis activity. The extract of C. lentillifera also enhanced phagocytosis activity of murine macrophage against E. coli. C. lentillifera extract enhanced activity of RAW 264.7 murine macrophage by increasing NO production and phagocytosis activity. This extract also caused suspected-giants’ cells as indicator of phagocytosis effect. Conclusion: This result provides an insight that the potency of C. lentillifera extract as immunostimulant.

    Key words: Caulerpa lentillifera, Immunomodulatory, Macrophage, Nitric oxide, Phagocytosis.

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