Influencing Effect of Heat Therapy on Osteoblasts Growth and Differentiation Following Treatment with Bone Antiresorptive Drugs- An in vitro Study

    Published on:November 2019
    Journal of Young Pharmacists , 2019; 11(4):395-398
    Original Article | doi:10.5530/jyp.2019.11.81

    Alqhtani NR1,*, Adel Alenazi1, Fazil Arshad Nasyam1, Sajeda Mehaji2

    1Department of Oral and Maxillofacial Surgery and Diagnostics Sciences, College of Dentistry, Prince Sattam Bin Abdul Aziz University, Alkarj, SAUDI ARABIA.

    2Department of Emeritus Reader in Oral Biology, Eastman Dental Institute University College-London, London, UNITED KINGDOM.


    Objectives: To find out the effect of mild heat shock on osteoblast proliferation and mineralisation following treatment with bisphosphonates. Materials and Methods: Cell culture of MC3T3 cells was performed in alpha minimum essential medium supplemented with 10% Foetal Calf Serum (FCS), 2 mmol L-glutamine and 100U/ml each of penicillin/streptomycin and were incubated at 37°C in humidified 5% CO2. The selected bisphosphonate drugs were administered, following which heat therapy was given by placing the flasks in a temperature-regulated water bath. The proliferation of osteoblast progenitors was measured using the methylene blue staining technique. Results: The results showed that mild heat-shock for 2 min at 42°C can stimulate proliferation of osteoblast progenitors, as well as bone nodule formation. In addition, mild heat-shock can reverse the action of bisphosphonate by stimulation of osteoblast proliferation and mineralisation. Conclusion: Mild heat-shock enhances cell differentiation and osteoblasts mineralisation following bisphosphonate treatment.

    Key words: Bisphosphonates, Heat-shock, Osteoblast, Osteoclast.

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