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Impacts of Nitrogen Starvation on Expression Profiles of Involving Genes in Triacylglycerol Biosynthesis in Chlamydomonas reinhardtii: Towards Microalgal Systems Biotechnology

1★, 2★ Corresponding author

  1. 1Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, IRAN.
  2. 2Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71468-64685, Shiraz, IRAN.

CORRESPONDENCE

Mohammad Hossein Morowvat

Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, IRAN.

mhmorowvat@sums.ac.ir

Received: 29-05-2017; Revised: 07-06-2017; Accepted: 31-07-2017.

Volume 9, Issue 4 · pp. 496-501 · 2017 · DOI: 10.5530/jyp.2017.9.97

ABSTRACT

Objective: Chlamydomonas reinhardtii, the most commonly studied model organism in microalgal kingdom, is known to be a rich lipid producer.Methods: Here, a naturally isolated C. reinhardtii strain was cultivated phototrophically at Erlenmeyer scale in BG11 culture medium. After reaching to stationary growth phase, a nitrogen limited regime with different nitrogen concentrations was applied.Results: Quantitative real time PCR analysis of DGTT1 and DGTT2 genes involved in triacylglycerol accumulation confirmed an increased level of expression for DGTT1 (21.05-fold increase) in nitrogen starvation compared with the nitrogen rich conditions. Besides, DGTT2 transcript levels showed a slight decrease in nitrogen deprivation condition (0.61-fold).Conclusion: The results opened a new way to direct microalgal systems biotechnology towards maximization of triacylglycerol production for green energy production.

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    Morowvat, M. H., & Ghasemi, Y. (2017). Impacts of Nitrogen Starvation on Expression Profiles of Involving Genes in Triacylglycerol Biosynthesis in Chlamydomonas reinhardtii: Towards Microalgal Systems Biotechnology. Journal of Young Pharmacists, 9(4), 496-501. https://doi.org/10.5530/jyp.2017.9.97

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    © 2017 Author(s)

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