Optimization of Microwave-Assisted Extraction of Active Com pounds, Antioxidant Activity and Angiotensin Converting En zyme (ACE) Inhibitory Activity from Peperomia pellucida (L.) Kunth

ABSTRACT

Introduction: to obtain the optimum extraction conditions of total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AA) and an giotensin converting enzyme (ACE) inhibitory activity from Peperomia pellucida (L.) Kunth.Method: TPC, TFC, AA and ACE inhibitory activity was measured by Folin-Ciocalteu method, AlCl3 method, DPPH and FRAP methods, and ACE kit WST. MAE efficiency parameters used were ethanol concentration, a sample to solvent ratio, extraction time, and microwave power.Results:The optimum conditions of MAE for TPC (49.78 mg GAE/g extract) and TFC (37.18 mg QE/g extract) were 80% ethanol, sample-solvent ratio of 1:12, extraction time of 2 min, MAE power of 30% and 80% ethanol, ratio of 1:12, time of 2 min, and 70% power respectively. The optimum AA was obtained at 65% ethanol, ratio of 1:10, time of 3 min, 50% power (DPPH method), and 65% ethanol, the ratio of 1:12, time of 1 min, and 70% power (FRAP method) respectively. The optimum ACE inhibitory activity (54.73% at a concentration of 100 µg/mL) were obtained at 80% ethanol, ratio of 1:12, time of 2 min, and 70% power.Conclusion: The analysis of Pearson correlation indicated that there was no correlation between TPC with FRAP method; TPC with ACE inhibitory activity and TFC with DPPH method.

KEYWORDS

• ACE
• Antioxidant Activity
• Flavonoid Compounds
• Microwave-As sisted Extraction
• Peperomia pellucida (L.) Kunth
• Phenolic Compounds

Showing references and in-text citations exactly as published.

• DOI10.5530/jyp.2017.1s.19Copy

Copyright and licence

© 2017 Author(s)

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0

RELATED ARTICLES

Matched on section and article type within this issue of JYP.

• Article · VOLUME 9, ISSUE 1SEffect of Using the Combination of Tween 80 and Ethanol on the Forming and Physical Stability of Microemulsion of Eucalyptus Oil as AntibacterialRahmah Elfiyani, Anisa Amalia, Septian Yudi Pratama · pp. s1-s4Original ArticleSame type: Article
• Article · VOLUME 9, ISSUE 1SUrinary Hydrogen Peroxide and Renal Function Parameter of Type 2 Diabetes Mellitus Patients Consuming Metformin and Metformin-SulfonylureaRani Sauriasari, Rizky Mutiara Mahani, Andisyah Putri Sekar, Azizahwati a · pp. s5-s8Original ArticleSame type: Article
• Article · VOLUME 9, ISSUE 1SAppropriate Use of Proton Pump Inhibitor in Inpatients of Cen tral Army Gatot Soebroto HospitalMeutia Anindita, Nadia Farhanah Syafhan, Yetti Hersunaryati, Retnosari Andrajati · pp. s9-s12Original ArticleSame type: Article
• Article · VOLUME 9, ISSUE 1SGastrointestinal Side Effects of Proton Pump Inhibitors on Inpa tients at Gatot Soebroto HospitalInas Fadhilah Hanif, Nadia Farhanah Syafhan, Yetti Hersunaryati, Retnosari Andrajati · pp. s13-s15Original ArticleSame type: Article