Differential Modulation of Circulating Endothelial Progenitor Cells in Peripheral Blood Monocyte Population of an Early Stage of Streptozotocin-Induced Diabetic Retinopathy

    Published on:April 2016
    Journal of Young Pharmacists, 2016; 8(3):198-205
    Original Article | doi:10.5530/jyp.2016.3.6
    Authors:

    Rupadevi Muthaian and Ramasamy Raveendran

    Department of Pharmacology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, INDIA.

    Abstract:

    Objective: To enumerate the circulating endothelial progenitor cells (EPCs) in peripheral blood mononuclear cells (PBMC) in streptozotocin (STZ) induced diabetic retinopathy (DR) rat model. Materials and Methods: Retinal microvascular complications in STZ induced diabetic Wistar rats were validated after 4 months using trypsin digestion assay of retinal vasculature, histological analysis of retina, glial fibrillary acidic protein (GFAP) immunostaining. Subsequently, circulating EPCs were quantified in PBMCs from diabetes induced rats after 4 months using markers such as CD-34, CD-31, CD-146, VE-Cadherin and Flk-1 by flow cytometry. Results: After 4 months of diabetes induction, pericyte loss, acellular capillaries, retinal layer thickness, microglial activation and increased nitric oxide (NO) levels were observed. While the EPC markers such as CD-34, CD-31, VE-Cadherin were decreased, the Flk-1+ve cells were up-regulated in the endogenous EPC pool. Conclusion: The quantity of EPCs are modulated in diabetes which may provide an insight into the use of EPCs as a biomarker for the progression of diabetes induced microvascular complication.

    Key words: Diabetic retinopathy, Endothelial progenitor cells, FlK-1, GFAP, Streptozotocin.

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