Published on: October 2009
Journal of Young Pharmacists, 2009; 1(4):292-295
Original Article | doi:10.4103/0975-1483.59330
Abstract:
The rapid, simple, and accurate chromatographic (high-performance liquid chromatography) and spectrophotometric method for the determination of progesterone in capsule was elaborated. Methanol was found to be a suitable extraction solvent. The samples were chromatographed on Linchrocart C18 column and UV detection at 254 nm. The elution was achieved isocratically with a mobile phase of methanol – water (80:20, v/v). The method was validated for precision, linearity, accuracy, and limit of detection.
Key words: Analysis in capsules, progesterone, reversed-phase chromatography, UV-spectrophotometry.
