Background: Ficus deltoidea is a medicinal plant known for treatment by ethnic Dayaks, Kalimantan and called Tabat Barito. Empirically, the majority of Tabat Barito leaves are used to treat candidiasis. Scientific data on F. deltoidea activity in its activity against candidiasis or candida are still minimal. Metabolite profiling from the most active fraction of F. deltoidea against Candida has not been reported. Objectives: This study aimed to explore the most active part of F. deltoidea leaves against C. albicans by obtaining the most active fraction and identification of compounds in the most active fraction through metabolite profiling using UPLC-QToF-MS/ MS. Methods: The leaves were macerated using solvents with an increase in polarity, fractionation was done by column chromatography, while antifungal activity assay was carried out by diffusion method. Metabolite profiling was done using LC-MS/MS. Results: Assay results of extracts from F. deltoidea leaves against C. albicans showed that the best activity was owned by ethyl acetate extracts, with an inhibition zone of more than 10 mm. The strongest activity against C. albicans was shown by FrEA5 with an inhibition zone of 30.67 ± 1.155 mm, stronger than Voriconazole as a positive control with inhibition zone: 19.75 ± 0.5 mm. The metabolites that can be identified in FrEA5 were Nigeglanine; 1,1,2,3,3-Pentamethylindane; and 1,1’-(1,1-Ethenediyl)bis(3-methylpiperazine). Conclusion: This research shows that FrEA5 is the most active fraction of F. deltoidea leaves against C. albicans and the compounds contained in FrEA5 include Nigeglanine; 1,1,2,3,3-Pentamethylindane; and 1,1’-(1,1-Ethenedyl)bis(3- methylpiperazine).
Key words: C. albicans, Ficus deltoidea, FrEA5, Metabolite Profiling, UPLC-QToF-MS/MS.