Objective: The aim of this study was to determine the effectiveness of buni-berry (Antidesma bunius) extract to increase PON1 mRNA expression in BALB/c mice fed with a high-fat diet. Methods: Twenty, thirty-weeks old male BALB/c mice were randomly allocated into four intervention groups; buni berry extract, simvastatin, control-sick and control-normal groups. All groups received high fat diet, except control-normal group which received control normal-diet. The diets were provided for 12 weeks. Treatments were started in all groups at the same time with the administration of the diets. Mice in buni-berry group were treated with oral buni-berry 300 mg/kgBW/day. In simvastatin group the mice were provided with oral simvastatin 6 mg/kkBW/day. The mice in control-sick and control-normal group were not received additional treatment. The intervention was conducted in 12 weeks. Mice body weight was measured weekly. Blood samples from tails were examined at the baseline and after12 weeks intervention for PON1 mRNA expression. Expression of PON1 mRNA was analysed by quantitative Real Time Polymerase Chain Reaction (qRT-PCR). Results: The changes mice body weights during interventions in all groups were not significantly different. PON1 mRNA expression was significantly increase in the buni berry extract group and simvastatin group, while in control-sick group the expression was significantly reduced. Conclusion: Oral administration of 300 mg/kgBW/day of buni-berry extract is effectively increases PON1 expression in BALB/c mice fed with a high fat diet.
Key words: Buni-berry extract, Antidesma bunius, PON1 mRNA, Atherosclerosis, High fat diet.
