Evaluation of the Antifungal Effect Vitis vinifera Extract on Candida albicans

    Published on:March 2018
    Journal of young pharmaciation, 2018; 10(2):164-168
    Original Article | doi:10.5530/jyp.2018.10.37

    Leandro Maciel Uchoa Gadelha1, Lídia Audrey Rocha Valadas1, Nicole de Mello Fiallos2, Sônia Luque Peralta3, Keniesd Sampaio Mendonça1, Mara Assef Leitão Lotif1, Gilvan Lima Bezerra4, Márcio Glauber Lopes de Aguiar1, Érika Sabóia Guerra Diógenes1, Edilson Martins Rodrigues Neto1, George Otávio Asevedo1, Ana Cristina de Mello Fiallos1

    1Pharmacy, Dentistry and Nursing College, Federal University of Ceara, Fortaleza, CE, BRAZIL.

    2Dentistry College State University of Rio de Janeiro, UERJ, Rio de Janeiro, RJ, BRAZIL.

    3State University of Ceara, UECE, Fortaleza, CE, BRAZIL.

    4Paulo Picanço College of Dentistry, Fortaleza, CE, BRAZIL.


    Objective: This research analyzed in vitro the antifungal activity of the solution composed by grapes seed extract Vitis vinifera (Doctor’s A-Z®) against Candida albicans (ATCC 20231) species aiming to determine the minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC). Methods: To that, were used micro dilution boards of 96 wells where the strain of Candida. Was tested the diluted solutions in distilled water on the concentrations 0.20 g/mL, 0.30 g/mL and 0.40 g/mL. As growing control were used growing fungous, to test the viability of the strains. As positive control, was used nystatin (commercial suspension 100,000 UI/mL) and as sterility control of growing way. The tests were conducted in triplicate and the data obtained were analyzed through the exam Kruskal-Wallis, with significance level of 95% (p<0.05). Results: The results indicated that all the samples were sensible to the analyzed extract and the CIM was obtained with the solution of 0.30 g/mL (30%). However, wasn’t observed a fungicide effect (MFC) on the tested concentrations. Conclusion: We conclude that the grapes seed extract can be an alternative against the gender Candida, however are necessary larger studies to prove their ant fungous action.

    Key words: Grapes seed extract, Stomatitis under prosthesis, Candida albicans, Dental prosthesis.

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