A simple, accurate, precise, economic, and speciÞ c stability indicating the RP-HPLC method has been developed for the estimation of Miglitol in tablet dosage form and spiked rabbit plasma. Chromatographic separation was achieved using Lachrom HPLC with Lichrospher, ODS, (250х 4.6) mm, ф 5 column at ambient temperature with 0.05 M ammonium acetate as a mobile phase at a ß ow rate of 0.5 ml/min and 216 nm was selected as a wave length for detection of a method. The retention time was 6.45 min. The standard curve was found to be linear (r2= 0.996) in the concentration range 800-1200 µg/ml using 1000 µg/ml as the test concentration. Apparent recovery was 98.338-101.704 % with RSD 0.942-0.964 for two brands. The method was repeatable with RSD 0.736. The intraday and interday precisions were RSD 0.957 and 1.019, respectively. LOD and LOQ of the method were 20 and 70 µg/ml, respectively. The presented study separates degradents and peaks appeared after spiking drug with plasma with a resolution of more than 1.5.
Key words: Forced degradation, miglitol, RP-HPLC, stability indicating.
