Formulation and characterization of Anti alzheimer’s drug loaded chitosan nanoparticles and its In vitro biological evaluation

Objective: The main objective of the study is to formulate hydrophilic drug loaded sustained release nanoparticles with the size of 200 nm and to increase the encapsulation efficiency of the drug. Methodology: The nanoparticles were prepared by simple ionic gelation method using various concentrations of chitosan and TPP. The prepared nanoparticles were evaluated for particle size, shape, charge, encapsulation efficiency, in vitro drug release and in vitro cytotoxicity. Results: The optimized drug loaded nanoparticles showed the size of 125 ± 4mn with PDI 0.25 ± 0.05, potential of +40 ± 2 mV, encapsulation efficiency of 65.5 ± 1.2% and the drug release of 68.4 ± 1.6% with an initial burst effect up to one hour followed by sustained release up to 24 hrs. Further the optimized formulation was subjected to investigate the cytotoxicity of CS-NP in SH-SY-5Y cell lines it revealed that the cell viability was above 90% without any toxicity. Conclusion: These preliminary results demonstrate that the possibility of delivering hydrophilic drugs to brain with enhanced encapsulation efficiency.

Key words: Chitosan, Hydrophilic drugs, Nanoparticles, Cytotoxicity, Blood brain barrier.