Anti-diabetic activities of (part) and fractions of Stereospermum tetragonum DC

Objectives: To identify the active principles, determine the anti-diabetes activity of fraction of Stereospermum tetragonum root. Materials and Methods: The efficacy was evaluated in streptozotocin induced type 2 diabetic rats and the anti-hyperglycemic activity was studied by glucose tolerance test. The major active compounds were isolated by solvent fractionation and chromatographic techniques and characterized with spectral data. Results: The active fraction of S. tetragonum showed presence of anti-diabetes mellitus activity in type-2 diabetic rats. It did not significantly influence insulin release from cultured islets. Two active principles (active at 2 mg/kg dose) were isolated and characterized with spectral data.One of themwas identified as an iridoid type glycoside and the other one was a lapachol like compound (derivative of naphthoquinone). Conclusions: Two active principles from the anti-diabetes fraction of S. tetragonum root were isolated and identified as an iridoid glycoside and a naphthoquinone derivative.

Key words: Iridoid glycoside, Naphthoquinone derivative, Stereospermum tetragonum, Anti-diabetes mellitus.