HPLC analysis and standardization of Brahmi vati e An Ayurvedic poly-herbal formulation

Objectives: The aim of the present study was to standardize Brahmi vati (BV) by simultaneous quantitative estimation of Bacoside A₃ and Piperine adopting HPLC-UV method. BV very important Ayurvedic polyherbo formulation used to treat epilepsy and mental disorders containing thirty eight ingredients including Bacopa monnieri L. and Piper longum L. Materials and methods: An HPLCeUV method was developed for the standardization of BV in light of simultaneous quantitative estimation of Bacoside A₃ and Piperine, the major constituents of B. monnieri L. and P. longum L. respectively. The developed method was validated on parameters including linearity, precision, accuracy and robustness. Results: The HPLC analysis showed significant increase in amount of Bacoside A₃ and Piperine in the inhouse sample of BV when compared with all three different marketed samples of the same. Results showed variations in the amount of Bacoside A₃ and Piperine in different samples which indicate nonuniformity in their quality which will lead to difference in their therapeutic effects. Conclusion: The outcome of the present investigation underlines the importance of standardization of Ayurvedic formulations. The developed method may be further used to standardize other samples of BV or other formulations containing Bacoside A₃ and Piperine.

Key words: Brahmi vati, Bacoside A₃, Piperine, HPLC.