RETRACTED: Apoptosis and Necrosis of Human Breast Cancer Cells by an Aqueous Extract of Euphorbia hirta leaves

    Published on:April/2016
    Journal of Young Pharmacists, 2016; 8(3):186-193
    Original Article | doi:10.5530/jyp.2016.3.4
    Authors:

    Bandana Behera1, Jyoshna Dash1, Debasish Pradhan1*, Gitanjali Tripathy2, Rakesh Pradhan1

    1Department of Pharmaceutical Sciences, Utkal University, Vani-Vihar, Odisha, INDIA.

    2Department of Pharmacy, Govt Polytechnic, Chandrasekharpur, Bhubaneswar, Odisha, INDIA.

    Abstract:

    Introduction: Traditional medicines for mammary tumour are unreasonable and have genuine symptoms. Non-ordinary normal medications have increased wide acknowledgment because of their assurance of a cure with negligible or no symptoms, however minimal experimental confirmation exists. One such basic cure is the leaves of the Euphorbia hirta plant. Methods: It is initially reported utilization of the fluid concentrate of Euphorbia hirta leaves breast cancer cells. The capacity of the concentrate to impel apoptosis and corruption in the human bosom malignancy cell line MCF-7, contrasted with typical human skin fibroblasts (MDA MB-231), was dictated by morphological changes in the cells utilizing light microscopy, DNA fragmentation, and brilliant stains (Annexin V and Propidium Iodide) utilizing Flow Cytometry and fluorescent microscopy. Results: Apoptosis was instigated in both cells, and more in MCF-7, when they were treated with 25% and half concentrate, while rot was watched mostly after presentation to raised concentrate fixations (75%). DNA discontinuity came about for both cells, in a period and dosage subordinate way. Both cells, at all concentrate fixations, demonstrated no critical contrasts in the quantity of living, dead, apoptotic, and necrotic cells. Conclusion: At long last, the outcomes might show that apoptotic changes in MCF-7 might be free of caspase-3, which is included in apoptosis and is inadequate in MCF-7 cells.

    Key words: Apoptosis, Aqueous extract, Euphorbia hirta leaves, Human breast cancer cells, Necrosis.

    This article has been retracted from Journal of Young Pharmacists on 16th December 2019. Retraction notice will be published in Jan-Mar, 2020 issue. 

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