Antioxidant, Antibacterial and Brine Shrimp Lethality Bioassay of Amoora cucullata, a Mangrove Plant

    Published on:Nov, 2015
    Journal of Young Pharmacists, 2016; 8(1):33-38
    Original Article | doi:10.5530/jyp.2016.1.8
    Authors:

    Raihana Pervin1*, Sadia Afrin1, Farah Sabrin2, Umme Salma Zohora3, Mohammad Shahedur Rahman3, Kazi Didarul Islam1 and Md. Morsaline Billah1

    1Natural Products Biotechnology Laboratory, Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh.

    2Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh.

    3Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar-1342, Bangladesh.

    Abstract:

    Objective: To determine antioxidant, antibacterial and brine shrimp lethality bioassay of Amoora cucullata using bark and leaf extracts of petroleum ether, chloroform and methanol. Methods: Bark and leaf parts were separately soaked into petroleum ether, chloroform and methanol respectively. To determine the presence of antioxidants; DPPH free radical scavenging assay, total phenolics and flavonoids determination and reducing power assay were carried out. Disk diffusion and brine shrimp lethality bioassay were conducted for the preliminary screening of antibacterial activity and cytotoxicity. Results: IC50 values in DPPH free radical scavenging assay for the extracts of petroleum ether, chloroform and methanol were found to be 316.23, 1192.42 and 128.82 in bark and 1106.32, 1330 and 25.12 μg/mL in leaf extracts respectively. Total phenolic content determined by Folin-Ciocalteu method in bark were 0, 0 and 58.41 mg gallic acid/gm of dry plant material in petroleum ether, chloroform and methanol while 8.11, 0 and 0 mg in leaf respectively. Total flavonoid content in bark and leaf was found to be 663.60, 549.47 and 46.25 mg and 1.64, 0 and 14.09 mg QE/gm of dried plant material in petroleum ether, chloroform and methanol respectively. In reducing power assay,methanolic extracts exhibited good reducing capacity. In antibacterial activity, extracts showed significant inhibition against enteropathogenic bacteria. Besides in brine shrimp lethality bioassay LC50 value was found to be 10, 2.301 and 7.28 in bark and 1.308, 1.94 and 2.14 μg/mL in leaf extracts of petroleum ether, chloroform and methanol respectively. Conclusion: From the present research work it can be concluded that A. cucullata leaf possess moderate antioxidant properties; strong antibacterial activity that correlates with their folkloric uses and potential cytotoxic properties.

    Key words: Antioxidant, Antibacterial, Cytotoxicity, Amoora cucullata.

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