Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina
1, 1★, 1,2★ Corresponding author
- 1Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, IRAN.
- 2Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71468-64685, Shiraz, IRAN.
CORRESPONDENCE
Mohammad Hossein Morowvat
Pharmaceutical Sciences Research Center, School of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, IRAN.
Received: 28-06-2016; Revised: 30-07-2016; Accepted: 07-08-2016.
Volume 9, Issue 1 · pp. 43-46 · 2016 · DOI: 10.5530/jyp.2017.9.9
ABSTRACT
Objective: Microalgal pharmaceutical biotechnology is mainly dependent on the biomass yield and also the final concentration of the obtained lipids. β-carotene is one of the most precious nutraceuticals, of both preventive and therapeutics importance in pharmacy and medicine. Dunaliella salina is known as famous β-carotene producer which could accumulate the β-carotene up to 10% of its dry cell weights. The amount of different macro and micronutrients in D. salina culture medium defines its productivity and β-carotene content.Methods: In this study, the effects of sulfur, iron and manganese deprivation, on cell growth and β-carotene biosynthesis in a naturally isolated strain of D. salina was examined. Besides, the fatty acid profile of the naturally isolated strain was also investigated.Results: Sulfur, iron and manganese deprivation caused a noticeable decrease in the cell growth of D. salina. On the other hand, in nutrient depleted media, the maximum β-carotene concentration was significantly improved (14.616 mg L-1 in sulfur starvation, 14.994 mg L-1 in iron starvation and 10.119 mg L-1 in manganese starvation media) compared with initial values (6.753 mg L-1) in basic culture medium. The obtained fatty acids from the studied micro algal strain found to be some important saturated, monounsaturated and polyunsaturated fatty acids.Conclusion: Owing to its significant growth rate, β-carotene contents and fatty acid profile; the naturally isolated micro algal strain could be exploited as a potential producer strain. Besides, the nutrient limitation strategy could be effectively employed to improve the β-carotene production procedure in D. salina.
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Shaker, S., Morowvat, M. H., & Ghasemi, Y. (2016). Effects of Sulfur, Iron and Manganese Starvation on Growth, β-carotene Production and Lipid Profile of Dunaliella salina. Journal of Young Pharmacists, 9(1), 43-46. https://doi.org/10.5530/jyp.2017.9.9
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