Journal of Young Pharmacists logo
Submit
Journal of Young PharmacistsACADEMIC PUBLISHING
Article

Capillary Electrophoresis Method Development for Simultaneous Determination of Atorvastatin and Ezetimibe from Solid Dosage Form

1★★ Corresponding author

  1. 1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, KSA.

CORRESPONDENCE

Mahesh Attimarad

Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, KSA.

mattimarad@kfu.edu.sa

Received: 22-06-2016; Revised: 19-07-2016; Accepted: 07-08-2016.

Volume 9, Issue 1 · pp. 120-123 · 2016 · DOI: 10.5530/jyp.2017.9.22

ABSTRACT

Purpose: A simple, rapid, validated capillary zone electrophoresis (CZE) was established for the simultaneous estimation of atorvastatin and ezetimibe from solid dosage form.Method: The separation of both the drugs was accomplished on fused silica capillary (48cm × 50µmi.d.) using background electrolyte consisting of 2mM borate buffer (pH 9.3) and methanol (80:20 v/v) as BGE. All the capillary conditions were optimized and newly developed method was validated as per the ICH guidelines.Results: Good linear calibration was obtained for both the analytes ranging from 5 to 200µg/ml and 2 to 20 µg/ml for atorvastatin and ezetimibe respectively. The limit of detection and quantification were less than 2 µg/ml for both the analytes. Repeatability and intra-day precision were well within the acceptable range. The accuracy was expressed as percent recovery, which is found to be between 98.20% to 100.41%.Conclusion:The newly developed simple and validated CZE method was used for simultaneous determina tion of atorvastatin and ezetimibe from solid dosage form.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Attimarad, M. (2016). Capillary Electrophoresis Method Development for Simultaneous Determination of Atorvastatin and Ezetimibe from Solid Dosage Form. Journal of Young Pharmacists, 9(1), 120-123. https://doi.org/10.5530/jyp.2017.9.22

    Copyright and licence

    © 2016 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0