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Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of Paracetamol and Flupirtine maleate in Pure and Pharmaceutical Dosage Forms

1★, 2★ Corresponding author

  1. 1Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh, INDIA.
  2. 2Department of Pharmaceutical Analysis & Quality Assurance, University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, INDIA.

CORRESPONDENCE

Mallikarjunarao Nagasarapu

Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh, INDIA.

mallimpharmmba@gmail.com

Volume 7, Issue 2 · pp. 81-88 · 2014 · DOI: 10.5530/jyp.2015.2.5

ABSTRACT

Objective: The objective of the proposed method was to develop a simple, fast, sensitive, and validated high-performance liquid chromatography (HPLC) method for the simultaneous estimation of Paracetamol and Flupirtine Maleate in combineddosage form.Materials and Methods: A Hypersil BDS C18, 150 x 4.6, 5 μ column with mobile phase containing Phosphate buffer (Ph 6.2): Acetonitrile (600:400) was used. The flow rate was 1.0 mL/min, column temperature was 30°C and effluents were monitored at 245 nm. The retention times of Paracetamol and Flupirtine Maleate were 3.1 min and 5.2 min respectively.Results: The correlation co-efficient for Paracetamol and Flupirtine Maleate were found to be 0.99 and 1 respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. Recovery of Paracetamol and Flupirtine Maleate in formulations was found to be 100% and 100% respectively confirms the noninterferences of the excipients in the formulation. Degradation studies reveals that purity threshold is greater than the purity angle hence the peak is said to be pure.Conclusion: Due to its simplicity, rapidness and high precision, this method was successfully applied to the estimation of Paracetamol and Flupirtine Maleate in combined dosage form.

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    Nagasarapu, M., & Dananna, G. (2014). Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of Paracetamol and Flupirtine maleate in Pure and Pharmaceutical Dosage Forms. Journal of Young Pharmacists, 7(2), 81-88. https://doi.org/10.5530/jyp.2015.2.5

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    © 2014 Author(s)

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