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Simultaneous Quantitative Analysis of Olmesartan Medoxomil and Amlodipine Besylate in Plasma by High-performance Liquid Chromatography Technique

1★, 2, 2, 3, 4, 5★ Corresponding author

  1. 1Department of Pharmaceutical Chemistry, Smt. Kishoritai Bhoyar College of Pharmacy, Nagpur, INDIA.
  2. 2J. L. Chaturvedi College of Pharmacy, MIDC, Nagpur, INDIA.
  3. 3Sharad Pawar College of Pharmacy, Wanadongari, Nagpur, INDIA.
  4. 4Department of Pharmaceutical Science, Rashtrasant Tukadoji Maharaj Nagpur University, Amravati Road, Nagpur, INDIA.
  5. 5Gurunanak College of Pharmacy, Nagpur, Maharashtra, INDIA.

CORRESPONDENCE

Shah SK

Department of Pharmaceutical Chemistry, Smt. Kishoritai Bhoyar College of Pharmacy, Nagpur, INDIA.

shah.sapan@rediffmail.com

Volume 4, Issue 2 · pp. 88-94 · 2012 · DOI: 10.4103/0975-1483.96622

ABSTRACT

A rapid, simple and sensitive high-performance liquid chromatography (HPLC) method has been developed for quantification of olmesartan medoxomil (OLM) and amlodipine besylate (AM) in plasma. The assay enables the measurement of OLM and AM for therapeutic drug monitoring with a minimum detectable limit of 2 ng mL. The method involves a simple, one-step extraction procedure and analytical recovery was above 50%. The separation was performed on an analytical 250 × 4.6 mm Eurospher 100-5 C18 column. The wavelength was set at 239 nm. The mobile phase was a mixture of acetonitrile:0.05 M ammonium acetate buffer. 0.1 mL triethylamine at pH 6.8 was selected at a flow rate of 1.0 mL/min. The calibration curve for the determination of OLM and AM in plasma was linear over the range 2-2500 and 8-10,000 ng/mL AM and OLM. The coefficients of variation for interday and intraday assay were found to be <15%. The method can be applied to a pharmacokinetic and pharmacodynamic study of OLM and AM in a combined dosage form.

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    SK, S., AJ, A., DP, K., SC, D., SK, A., & SR, Y. (2012). Simultaneous Quantitative Analysis of Olmesartan Medoxomil and Amlodipine Besylate in Plasma by High-performance Liquid Chromatography Technique. Journal of Young Pharmacists, 4(2), 88-94. https://doi.org/10.4103/0975-1483.96622

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    © 2012 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0