Development and Validation of Microbiological Analytical Method for Determination of Potency of Voriconazole Tablets

    Published on:July 2020
    Journal of Young Pharmacists, 2020; (12):s113-s116
    Original Article | doi:10.5530/jyp.2020.12s.58
    Authors:

    Mami Sahitya1, Sana Fathima1*, Ancha Divya1, Kyadari Vaishali1, Meshkhana NidhiSree1, Challoori Spandana Reddy1, Toleti Sree Ramya1, Sandhya Rani1, Yenumula Padmavathi1, Ravi Kiran2

    1Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Hyderabad, Telangana, INDIA.

    2Department of Biotechnology, G. Pulla Reddy College of Pharmacy, Hyderabad, Telangana, INDIA.

    Abstract:

    A new microbiological method was developed for analysis of voriconazole tablets using Candida albicans as test microorganism. Various media, species and conditions were used to optimize the diffusion test. A prospective validation of the system showed adequate linearity (0.995), accuracy (RSD<2%) and consistency (mean recovery= 101.77%). High performance liquid chromatography was selected as a tool of comparison for evaluating voriconazole. Results of both the microbiological and HPLC methods have been compared with the student t-test and the voriconazole content determined by both methods has shown strong correlation. The developed microbiological analytical method provides true indication of biological activity and can be used in dosage forms for routine quality control analysis of voriconazole.

    Key words: Candida albicans, Comparison, Diffusion method, HPLC, Method validation, Voriconazole.

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