Introduction: Ginger (Zingiber officinale Rosc.) and sappan wood (Caesalpinia sappan L.) have been shown to inhibit platelet aggregation in vitro. Objective: This study aimed to prove the efficacy of the combination of both these plants by examining in vivo antithrombotic activity by the acute pulmonary thromboembolism model in mice experimentally. Materials and Methods: Mice were divided into two experimental groups (bleeding time and survival rate). Acute pulmonary thromboembolism was induced after a combination of ginger and sappan wood extract pretreatment, by an intravenous injection of the mixture of a collagen and epinephrine. The paralyzed and dead mice in each group were observed and the percentage of the protective effect against thromboembolism (survival rate) was calculated. Bleeding time was observed on mice tail that had been cut. Results: The Combination of ginger and sappan wood extract at a dose of 56 mg : 14 mg/20 g/day orally, showed an antithrombotic activity due to preventing the paralysis or death (83.33%) induced by collagen-epinephrine intravenous injection. The Combination of ginger and sappan wood extract at the same dose also extending the bleeding time of mice significantly (p ≤ 0.05) compared with the normal control. Conclusion: Based on these results, a combination of ginger– sappan extract has a potential effect as an antithrombotic agent.
Key words: Antithrombotic, Ginger, Sappan wood, Bleeding time, Survival rate.