Polyclonal T Lymphocytes Transform into Malignant Cells by Retroviral Mediated Transfer of p21SNFT Gene

    Published on:April 2020
    Journal of Young Pharmacists, 2020; 12(1):71-74
    Original Article | doi:10.5530/jyp.2020.12.14
    Authors:

    Ashok Kumar1,*, Bharathi Sengodan1, Chetan Lal2, Anthony Leela3, Kevin Fernandez3

    1Department of Pathology, Faculty of Medicine, AIMST University, MALAYSIA.

    2Department of Microbiology, Federal Postgraduate Medical Institute, Lahore, Punjab, PAKISTAN.

    3Department of Community Medicine, Faculty of Medicine, AIMST University, MALAYSIA.

    Abstract:

    Background: p21SNFT (21 kDa small nuclear factor isolated from T cells) gene is known to be increasingly expressed in the cells of Hodgkin disease and Hodgkin cell lines. It was not investigated before if this gene had any effect on polyclonal T lymphocytes. For this purpose, we transduced this gene into polyclonal T lymphocytes and transplanted into mice. Materials and Methods: We used γ-retroviral vector to transduce this gene into polyclonal T lymphocytes, isolated from wild type black-6 mice. After confirmation of p21SNFT expression via marker gene eGFP (enhanced green fluorescent protein) and Western blot analysis, these cells were transplaned into immunocompromised Rag 1 deficient mice. The control group of mice was transplanted with γ-retroviral vector, carrying only marker gene (eGFP). The mice were followed up. The results showed that all 7 mice transplanted with p21SNFT-carrying polyclonal T lymphocytes developed massively enlarged mesenteric lymph nodes after 435 to 550 days of transplantation. Results: Histopathological analyses of these lymph nodes revealed the lymphoma, which resembled human peripheral T cell lymphoma type. Furthermore, immunophenotyping of tumor cells by FACS analysis revealed loss of T cell markers and expression of CD19 marker. From the control group, all five mice remained healthy and did not develop any tumor. Conclusion: The study concludes that γ-retroviral mediated transfer of p21SNFT transforms polyclonal T lymphocytes into malignant cells.

    Key words: γ-retroviral vector, Lymphoma, Malignant transformation, p21SNFT, T lymphocytes.

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