Anti-cancer Activity and Gene Expression Responses to Methanol Extract of Gladiolus segetum in THP-1 Human Monocytic Leukemia Cells

    Published on:December 2018
    Journal of Young Pharmacists, 2019; 11(1):xx-xx
    Original Article | doi:10.5530/jyp.2019.11.x
    Authors:

    Marref Salah Eddine1,*, Benkiki Naima1, Melakhessou Mohamed Akram1, Olivier Joubert2, Zahra Manel Doumandji2, Ramia Safar2

    1Laboratoire de Biotechnologie des Molécules Bioactives et de la Physiopathologie Cellulaire. Université de Batna-2, 05000, ALGERIE.

    2Faculté de Pharmacie, EA 3452 CITHÉFOR, Université de Lorraine, 5 rue Albert Lebrun, 54000 Nancy, FRANCE.

    Abstract:

    Objective: to evaluate the viability of cells against exposure to the methanol extract of Gladiolus segetum (MEGS) by various colorimetric detection tests (Alamar Blue, WST-1) and the trypan blue exclusion test in Human Leukemia Monocytes THP-1 and Using reverse transcription-polymerase chain reaction assays. Methods: human THP-1 monocytic cell line was exposed to 25 to 800 μg/mL concentrations of MEGS for 24 h and cellular viability was estimated using Alamar Blue, WST-1 and trypan blue tests. Gene expressions were performed using reverse transcription-polymerase chain reaction assays and the measurement of Caspase-3 enzyme activity. Results: a decrease of viability was observed with a dose-dependent effect of MEGS on THP-1.In addition, a differential expression involved in the different genes tested. Moreover, it has been shown overexpression of casp3 for an exposure to 25 μg/ml and 100 μg/ml of MEGS. Conclusion: Our results show that the MEGS present a toxicity to THP-1 cells, especially with regard to apoptosis processes.

    Key words: Gene expressions, Viability, Gladiolus segetum,THP-1, Toxicity.

     

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