Effect of Treatment with Conjugated Linoleic Acid on the Enzyme Carnitine Palmitoyl Transferase in Albino Mice

    Published on:October 2018
    Journal of Young Pharmacists, 2018; 10(4):418-421
    Original Article | doi:10.5530/jyp.2018.10.92

    Bhyravavajhula Lalitha Devi1*, Muthiah Nagasundaram2, Kasturi Satyanarayana Murthy3, Chintada Ganapati Swamy4

    1Research Scholar, Bharath University, West Tambaram, Chennai, Tamil Nadu, INDIA.

    2Professor of Pharmacology, Sri Balaji Medical College, Chennai, Tamil Nadu, INDIA.

    3Professor of Pharmacology, GSL Medical College, Rajamahendravaram, Andhra Pradesh, INDIA.

    4Assistant Professor of Biostatistics, Department of Community Medicine, GSL Medical College, Rajamahendravaram – 533296, Andhra Pradesh, INDIA


    Background: Conjugated Linoleic Acid is an omega-6 fatty acid with potential health benefits. The objective of the present study is to study the effect of Conjugated Linoleic Acid on the enzyme Carnitine Palmitoyl Transferase. Methods: Healthy Albino mice were divided into three groups viz., first group of 10 animals given high fat diet, second group of high fat diet and Conjugated Linoleic Acid treated animals and third group of control animals given normal diet. After 22 days, animals were sacrificed and hepatic and adipose tissue separated. Carnitine palmitoyl transferase estimation was done by ELISA and western blot techniques. Serum samples collected on 7th, 15th and 22nd days and carnitine palmitoyl transferase assay done. Results: The results showed that there is an elevation of the levels of the enzyme Carnitine Palmitoyl Transferase in serum, hepatic and adipose tissues as measured by both ELISA and Western blot technique. Conclusion: Conjugated Linoleic Acid, acts via elevation of the enzyme Carnitine Palmitoyl Transferase when it is given in mice and causes weight reduction.

    Key words: Omega-6 fatty acid, Albino mice, Elisa, Western blot technique.

    Article Download