Objective: The aims of this study were to know the safety using Hen’s eggs-chorioallantoic membrane (HET-CAM) test method and hair growth promoting activity of the ethyl acetate fraction of Nothopanax scutellarium leaves. Methods: Safety test was measured by scoring and categorizing irritation onHET-CAM. Meanwhile, activity test was conducted by applying the hair tonic of the ethylacetate fraction on the back of the rabbits, and the length of hair was measured in the 1st, 2nd and 3rd week.In the 3rd week, the hair growth was weighed and hair diameter also was measuredusing scanning electron microscope (SEM). Results: The result showed that 0.2 gram of ethyl acetate fraction of N.scutellarium leaves have mild irritation effect, whereas the formulation contained with 0.5% and 1% of fraction increased hair growth and hair diameter. Conclusion: The ethyl acetate fraction of N. scutellarium have mild irritan effect, and the hair tonic demonstrated hair growth promoting activity.
Key words: Hair Tonic, Hair Growth Activity, Nothopanax scutellarium, Safety, HET-CAM.